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International Poster Journal of Dentistry and Oral Medicine
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Int Poster J Dent Oral Med 10 (2008), No. 4     15. Dec. 2008

Int Poster J Dent Oral Med 2008, Vol 10 No 04, Poster 429

Periodontitis: Possible role of Mitochondrial DNA Mutations

Language: English
 

Authors:
Dr Rampalli Viswa Chandra, MDS DNB, Aileni Amarendhar Reddy, MDS,
SVS Institute of Dental Sciences, Yenugonda, Mahabubnagar, Andhra Pradesh, India
Periyasamy Govindaraj, MSc, Ayyasamy Vanniarrajan, PhD, Lalji Singh, PhD, Kumarasamy Thangaraj, PhD,
Centre for Cellular and Molecular Biology, Hyderabad, India

Date/Event/Venue:
December 6-8, 2007
International Symposium on Dissecting the Role of Genes and Environment in Complex diseases
Centre for Cellular and Molecular Biology, Hyderabad, India.
 

Introduction

Mitochondrial DNA mutations have been well documented in several human diseases. Mitochondrial structures are susceptible to oxidative damage by induced ROS, which are generated continuously by the mitochondrial respiratory chain. Mitochondria are also major sites for the accumulation of low molecular weight Fe2+ complexes, which promote the oxidative damage.Thus, it leads to mitochondrial dysfunction by damaging mitochondrial DNA (mtDNA), and this mtDNA damage is associate with aging process. There is no comprehensive study on the impacts of mtDNA mutations in Periodontotitis. Hence, we initiated this study, to understand the possible association of mtDNA in causing Periodontotitis.
 

Fig. 1
 
Fig. 2
 
Fig. 3
 
Fig. 4
 

Objectives

Possible association between Mitochondrial DNA mutations and chronic periodontitis.
 

Material and Methods

Clinical evaluation of a sample pool and the patients fulfilling the inclusion criteria were selected.Sample collections: attached gingiva was collected during periodontal surgery from 30 patients. On the samples, DNA isolation, Polymerase chain reaction, Complete sequencing of mtDNA, Sequencing analysis and Comparative genomics were performed.
 

Results

We found 264 variants/mutations across the mtDNA. A total of 16 novel mutations were found of which 3 were novel missense mutations and remaining 13 were polymorphic.
Two were heteroplasmic mutations. Eight mutations were reported to be associated with other mitochondrial disorders. Evolutionary analysis of these patients showed diverse mtDNA haplogroup background.
 

Fig. 5: A total of 16 novel mutations were found of which 3 were novel missense mutations and remaining 13 were polymorphic.
 
Fig. 6: Evolutionary analysis of these patients showed diverse mtDNA haplogroup background.
 

Conclusions

Mutations were observed in different genes across the mitochondrial genome. Haplogrouping revealed that the periodontitis occur at different ethnic backgrounds.
Present study suggests the possible role of mitochondrial DNA in periodontitis, however more samples from different ethnic background is needed to provide additional evidence for the role of mtDNA variation in periodontitis.
 

Literature

  1. Chapple ILC: Reactive oxygen species and antioxidants in inflammatory diseases. Journal of Clinical Periodontology 1997, 24, pp.287-296.
  2. Canakçi CF., Tatar A., Canakçi V., Cicek Y., Oztas S., Orbak R: New evidence of premature oxidative DNA damage: mitochondrial DNA deletion in gingival tissue of patients with periodontitis. Journal of Periodontology 2006, 77, pp.1894-1900.
  3. Tomofuji T., Azuma T., Kusano H., Sanbe T., Ekuni D., Tamaki N., Yamamoto T., Watanabe M: Oxidative damage of periodontal tissue in the rat periodontitis model: effects of a high-cholesterol diet. FEBS Letters 2006, 580, pp.3601-4.
     

Abbreviations

mtDNA = Mitochondrial DNA
rRNA = Ribosomal RNA
ND = NADH dehydrogenase
CO = Cytochrome c oxidase
ATPase = ATP synthase
tRNA = Transfer RNA
 

This Poster was submitted by Dr Rampalli Viswa Chandra.
 

Correspondence address:
Dr Rampalli Viswa Chandra
Senior Lecturer, Department of Periodontics
SVS Institute of Dental Sciences
Yenugonda
Mahabubnagar
Andhra Pradesh, India
Phone: 91-8542-271515
Fax: 91-8542-273111