Int Poster J Dent Oral Med 2008, Vol 10 No 04, Poster 429
Periodontitis: Possible role of Mitochondrial DNA Mutations
Dr Rampalli Viswa Chandra, MDS DNB, Aileni Amarendhar Reddy, MDS,
SVS Institute of Dental Sciences, Yenugonda, Mahabubnagar, Andhra Pradesh, India
Periyasamy Govindaraj, MSc, Ayyasamy Vanniarrajan, PhD, Lalji Singh, PhD, Kumarasamy Thangaraj, PhD,
Centre for Cellular and Molecular Biology, Hyderabad, India
December 6-8, 2007
International Symposium on Dissecting the Role of Genes and Environment in Complex diseases
Centre for Cellular and Molecular Biology, Hyderabad, India.
Mitochondrial DNA mutations have been well documented in several human diseases. Mitochondrial structures are susceptible to oxidative damage by induced ROS, which are generated continuously by the mitochondrial respiratory chain. Mitochondria are also major sites for the accumulation of low molecular weight Fe2+ complexes, which promote the oxidative damage.Thus, it leads to mitochondrial dysfunction by damaging mitochondrial DNA (mtDNA), and this mtDNA damage is associate with aging process. There is no comprehensive study on the impacts of mtDNA mutations in Periodontotitis. Hence, we initiated this study, to understand the possible association of mtDNA in causing Periodontotitis.
Possible association between Mitochondrial DNA mutations and chronic periodontitis.
Material and Methods
Clinical evaluation of a sample pool and the patients fulfilling the inclusion criteria were selected.Sample collections: attached gingiva was collected during periodontal surgery from 30 patients. On the samples, DNA isolation, Polymerase chain reaction, Complete sequencing of mtDNA, Sequencing analysis and Comparative genomics were performed.
We found 264 variants/mutations across the mtDNA. A total of 16 novel mutations were found of which 3 were novel missense mutations and remaining 13 were polymorphic.
Two were heteroplasmic mutations. Eight mutations were reported to be associated with other mitochondrial disorders. Evolutionary analysis of these patients showed diverse mtDNA haplogroup background.
|Fig. 5: A total of 16 novel mutations were found of which 3 were novel missense mutations and remaining 13 were polymorphic.
|Fig. 6: Evolutionary analysis of these patients showed diverse mtDNA haplogroup background.
Mutations were observed in different genes across the mitochondrial genome. Haplogrouping revealed that the periodontitis occur at different ethnic backgrounds.
Present study suggests the possible role of mitochondrial DNA in periodontitis, however more samples from different ethnic background is needed to provide additional evidence for the role of mtDNA variation in periodontitis.
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mtDNA = Mitochondrial DNA
rRNA = Ribosomal RNA
ND = NADH dehydrogenase
CO = Cytochrome c oxidase
ATPase = ATP synthase
tRNA = Transfer RNA
This Poster was submitted by Dr Rampalli Viswa Chandra.
Dr Rampalli Viswa Chandra
Senior Lecturer, Department of Periodontics
SVS Institute of Dental Sciences
Andhra Pradesh, India