Int Poster J Dent Oral Med 2008, Vol 10 No 02, Poster 398
Isolation of Candida Spp. in Dental Plaque of ECC Affected Children
Prof. MUDr. Martina Kukletová, Csc., MUDr. Jarmila Kuklová,
Stomatological Clinic, Faculty of Medicine, Masaryk University, Brno, Czech Republic
MUDr. Filip Ruzicka,
Teaching Hospital of. St.Ann, Brno
RNDr. Ivo Sedlácek, Csc.,
Faculty of Sciences, Masaryk University, Brno
MUDr. Lenka Zácková,
Children Teaching Hospital, Brno
June 13 - 17, 2007
21st Congress of IAPD
The most important fungal pathogen in the oral cavity are yeasts of Candida genus. It is a common yeast living in the oral cavity of about one-half of population. Yeasts are noted for their ability to ferment monosaccharides and some disaccharides to ethanol and carbon dioxide. Yeasts were also found in the dental plaque.
The study was carried out to identify yeasts isolated from the dental plaque samples obtained from children affected with ECC.
Material and Methods
Isolated yeast colonies obtained from the dental plaque smears cultivated on MRS agar at 37°C in 5% CO2 atmosphere (N = 58) were characterized. To quantify yeasts, further samples of dental plaque (N = 30) were smeared using sterile cotton swabs. The swabs were rinsed in 1ml of saline, 100µl of the solution were inoculated on Sabouraud dextrose agar and cultivated at 30°C. The number of colony-forming units was calculated and assessed as strong positive (+++), positive (++) and weakly positive (+). The species identification was established using morphological characteristics, characteristic growth on CHROMagar Candida, assimilation and fermentation tests (kits Auxalor 2 and ID 32C). Selected samples of cultivated colonies ( N = 10) were investigated in a scanning (SEM) electron microscope. Extracted primary teeth covered by dental plaque were investigated under SEM (N = 20) and a transmission (TEM) electron microscope (N = 10).
Among isolates cultivated originally on MRS agar 58 yeast strains were identified as
Candida albicans (51 strains), Candida tropicalis (4 strains), Candida dubliniensis (2 strains) and Rhodotorula rubra (1 strain). In 30 samples evaluated quantitatively Caldida albicans was found to be strongly positive in 5 samples, positive in 7 samples and 2 samples were weakly positive as well as 1 sample of Candida sp. and 1 sample of Candida guilliermondii. 14 samples were found to be negative.
SEM and TEM investigations confirmed the presence of Candida albicans, both hyphal and budding forms were revealed.
Table 1: Isolates cultivated on MRS agar (N=58)
|Type of the isolate
||Number of strains
Table 2: Quantitative detection and identification of Candida strains (N=30) from ECC affected children
|Type of the isolate
||Number of samples
|+ = weakly positive ++ = positive +++ = strongly positive
Negative results were obtained in control samples from caries-free children (N=3)
|Fig. 1a. Large area of the tooth surface covered by dental plaque of different morphology.
||Fig. 1b. Higher magnification of the area revealed a yeast-like microflora
||Fig. 1c Higher magnification demonstrated the dental plaque composed of Candida albicans
|Fig. 2. Cultivated colony of Candida albicans from dental plaque smears
||Fig. 3. Hyphal form of Candida albicans from dental plaque
||Fig. 4. Budding form of Candida albicans from dental plaque
Candida albicans and further yeast strains are constant components of dental plaque in ECC affected children and they can contribute by their carbohydrate fermenting ability to the destructive course of the disease.
The study was supported by Project 1M0528 from the Czech Ministry of Education.
- Marsh P., Martin M.V.: Oral Microbiology, Wright, 2001, p. 153-162
- Samaranayake L.P.: Essential Microbiology for Dentistry, Churchill Livingstone, 2002, p. 142-147, 239-250
ECC = early childhood caries
TEM = transmission electron microscope
SEM = scanning electron microscope
This Poster was submitted by Prof. MUDr. Martina Kukletová.
Prof. MUDr. Martina Kukletová, Csc.
Faculty of Medicine, Masaryk University
656 91 Brno