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International Poster Journal of Dentistry and Oral Medicine
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Int Poster J Dent Oral Med 8 (2006), No. 3     15. Sep. 2006

Int Poster J Dent Oral Med 2006, Vol 8 No 03, Poster 323

Different growing of osteoblasts on 3D structures in comparison with industrial and individual materials

Language: English

Authors:
Bettina Hohlweg-Majert, Department of Oral and Maxillofacial Surgery, Albert-Ludwigs-University, Freiburg
Prof. Dr. Dr. Rainer Schmelzeisen, Head of Department of Oral and Maxillofacial Surgery, Albert-Ludwigs-University, Freiburg
Ute Hübner, Department of Oral and Maxillofacial Surgery, Albert-Ludwigs-University, Freiburg
Dr. Nassrin Kiapur N1, Department of Oral and Maxillofacial Surgery, Albert-Ludwigs-University, Freiburg
Dr. Mario Wagner, Maxillofacial Surgery, University Hospital Freiburg
Rüdiger Landers, Department of Macromolecular Chemistry, University Hospital Freiburg
Prof. Dr. Rolf Mülhaupt, Department of Macromolecular Chemistry, University Hospital Freiburg
Prof. Dr. Dr. Nils-Claudius Gellrich, Department of Oral and Maxillofacial Surgery, University Hospital Freiburg

Date/Event/Venue:
03. - 06.09.2003
2nd European Tissue engineering
Genova

Introduction

In reconstructive surgery of maxillofacial defects the application of tissue engineering cell carrier matrices of bone grafts is needed. There is still a variety of indications for special supporting stability materials e.g. for calvarium reconstruction, sinus floor elevation and tumor surgery. The materials differ in pore size, composition, permeability, durability and pore density. Industrial and individual methods exist of producing such materials. The individual technique of rapid prototyping allows a three dimensional growing of cells.

Material and Methods

Scaffolds produced by rapid prototyping (PLGA, Resomer RG 503® Boehringer Ingelheim, Germany (Fig 1)) and one industrial produced material (Tissue FoliE, Baxter, native equine collagen) were compared. Both materials were seeded with 1 x 105 cells/ml osteoblasts. The cells were cultivated of corticolamellar bone of the maxilla from ten different patients and twelve sheep. After one week incubation (37°C / 5% CO2 atmosphere) cell proliferation test (EZ4U, Biomedica Vienna) was performed. Furthermore cell growing was investigated by using scanning electron microscope.

Fig.1: Individual PLGA scaffold produced by rapid prototyping

Fig.2: Scanning electron microscopy study of industrial and individual materials. On the left side (Fig A,C) are the collagen matrices and on the right side (Fig B,D) are the PLGA scaffolds produced by rapid prototyping. Only human osteoblasts are shown. They were cultivated and seeded with 1x105 osteoblasts/ml on each

Fig 3: Cell proliferation analysis of human (n=10) and sheep (n=12) osteoblasts seeded on individual PLGA and industrial collagen material. Little difference in cell proliferation between the materials for sheep osteoblasts exist. The difference between human and sheep osteoblasts on PLGA was obvious. The optimal growing conditions were on the industrial material with human osteoblasts.

Results

Comparing these experiments human osteoblasts showed a higher cell proliferation than sheep osteoblasts on each material. Furthermore the growing of sheep osteoblasts was better on PLGA 3 D plotted structure. There was a significant higher cell proliferation of human osteoblasts on the collagen matrix than on PLGA.

Conclusions

This study showed that both materials are equal with regard to cell proliferation and cell cytotoxic effects. The industrial and the individual structures are suitable for clinical application. The advantage of rapid prototyping is the possibility of producing individual scaffolds which allows three dimensional growing condition.

Literature

  • How to optimise seeding and culturing of human osteoblast-like cells on various biomaterials, Wiedmann-Al-Ahmad M, Gutwald R, Lauer G, Hübner U, Schmelzeisen R, Biomaterials 23(2002) 3319-3328
  • Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering, Landers R, Hübner U, Schmelzeisen R, Mülhaupt R, Biomaterials 23 (2002) 4437-4447

 

This Poster was submitted by Bettina Hohlweg-Majert

Correspondence address:
Bettina Hohlweg-Majert
Department of Oral and Maxillofacial Surgery
Albert-Ludwigs-University
Hugstetterstr. 55
79106 Freiburg
Germany