Int Poster J Dent Oral Med 8 (2006), No. 2 15. June 2006
Int Poster J Dent Oral Med 2006, Vol 8 No 02, Poster 312
SEM in the Studies on Dentine Adhesives
Prof. MUDr. Martina Kukletová, CSc., Stomatological Clinic, Medical Faculty, Masaryk University, Brno
Prof. MUDr. Drahomír Horký, Dept. of Histology and Embryology, Medical Faculty, Masaryk University, Brno
Ass. Prof. MUDr. Zdenka Halacková, CSc., Stomatological Clinic, Medical Faculty, Masaryk University, Brno
September 4-7, 2005
43rd Congress of the Czech Anatomical Society
The development in the area of tooth-colored restorative materials is closely linked with the discovery that resin materials could be bound to acid-etched enamel. The studies on bonding dentine were soon performed, and they made possible to anchor a composite filling material to dentine, too. In the course of time, 6 generations of adhesive systems were developed. At present, two main techniques have been used: total-etched and self-etching. The studies on the character of a connection between dentine and an adhesive material can be conducted by different morphological methods. The aim of the paper presented was to compare different SEM techniques and to determine the most suitable method for studying the dentine adhesion.
New materials have been introduced to esthetic dentistry, and their properties and connection to hard dental tissues are verified by different morphological studies. The problem may be a choice of the most suitable method for a given research.
Material and Methods
Sixteen teeth extracted for orthodontic reasons were used for the trial. The teeth were kept in 10% neutral formaline. The cavities of the Ist and Vth classes were prepared, and their surfaces were treated with dentine adhesives. Eight teeth were treated with total-etch technique using Prime bond or Single bond, and 8 teeth were treated with self-etching technique using Xeno III or Prompt-L-Pop materials. The teeth were fractured, and the fractured surfaces were studied in SEM. One-half of each tooth was studied under low-vacuum technique (LowVac) using BSE and LVSTD detectors, the other was gold-coated and investigated under high vacuum (HiVac) using SE and BSE detectors.
|Fig. 1. Total-etch technique
||Fig. 2. Self-etching technique
In both techniques a good connection between dentine and adhesive materials was found. A hybride layer was formed, and the tags of resin penetrated into the dentinal tubules. In total-etch technique longer tags were observed regularly. HiVac technique provided a sharper and more distinct picture of the situation and enabled the studies in greater details. No substantial differences between the applied detectors were found.
Scanning electron microscopy is frequently used in the research in various fields of dentistry (Nakabayashi and Pashley 1998, Hasshoff, Schneider and Merte 2004, Tsuchia et al. 2004, Wang and Spenser 2004). The technique for a sample preparation is relatively easy, and large areas of tissue under interest may be studied. Investigation in SEM is performed under high vacuum, and the samples have to be metal coated. Recently, a new technique working under low vacuum has been introduced into scanning electron microscopy. This low vacuum environment is useful for the three-dimensional analysis of the surface morphology of biological samples (Ushiki et al 1998). Soft and hard tissues can be studied under low vacuum conditions (Bonnema et al. 1997, Jeng et al 1999, Erwin 2003, Al-Qahtani et al 2003, Shipper et al 2004). Shipper et al (2004) studied marginal adaptation of mineral trioxide agregate and amalgam under LowVac versus HiVac and reported that the best results were achieved under LowVac investigation. Our previous study on adhesive systems (Kukletová et al 2005) has demonstrated that LowVac technique can distinguish different materials used while gold coated HiVac technique masked that connection. The present study, however, found the HiVac technique more suitable for the detailed investigation of dentine adhesion and it will be preferred in further studies.
The study was supported by IGA grant No 8055-3 from the Czech Ministry of Health.
- Al Qahtani,M.Q., Platt, J.A., Moore,B.K., Cochran, M.A.: The effect on shear bond strength of rewetting dry dentin with two desensitizers. Oper.Dent. 2003, 28(3), 287-296
- Bonnema, J., van Geel, A.N., Ligtenstein, D.A., Schmitz, P.I., Wiggers, T.: A prospective randomized trial of high versus low vacuum drainage after axillary dissection for brest cancer. Am.J.Surg. 1997, 173 (2), 76-79
- Erwin, M.H.: A simple low-vacuum environmental cell. Microsc.Microanal. 2003, 9 (1), 18-28
- Hasshoff, G., Schneider, H., Merte, K.: Tooth-composite interface from specific and nonspecific adhesive restorative systems. J.Biomed.Mat. Res.-Part B Applied Biomat. 2004, 71 (1), 38-45
- Jeng, J.H., Chen, K.W., Lin, C.P., Chou, H.G., Lan, W.H.: Ultrastructural changes of the tooth root surface by Nd:YA G laser irradiation followed by citric acid and tetracycline. J.Formos Med. Assoc. 1999, 98 (4), 242-247
- Kukletová, M., Kuklová J., Horký D., Halacková Z.: Adhesive systems in morphological picture. I. Tetric Ceram HB.(Czech) Prakt.zub.Lék. 2005, 53 (3), 37-41
- Nakabayashi, N., Pashley, D.H.:Hybridization of dental hard tissues. Tokyo: Quintessence publishing Co, 1998, 57-83
- Shipper, G., Grossmann, E.S., Botha, A.J., Cleaton-Jones, P.E.: Marginal adaptation of mineral trioxide agregate (MTA) compared with amalgam as a root-end filling material: a low-vacuum (LV) versus high-vacuum (HV) SEM study. Int.Endod. J., 2004, 37 (5), 325-336
- Tsuchiya, S., Nikaido, T., Sonoda, H., Foton, R.M., Tagami,J.: Ultrastructure of the dentin-adhesive interface after acid-base challenge. J.Adhesive Dent. 2004, 6 (3), 183-190
- Ushiki, T., Hashizume, H., Itoh, S., Kuboki, K., Saito,S., Tanaka, K.: Low-voltage backscattered electron imaging of non-coated biological samples in a low-vacuum environment using a variable-pressure scanning electron microscope with a YAG-detector. J.Electron Microsc.(Tokyo), 1998, 47 (4), 351-354
- Wang, Y., Spenser P.: Overestimated hybrid layer duality in polished adhesive/dentin interfaces. J.Biomed.Mat.Res. - Part A, 2004, 68 ((4), 735-746
SEM = scanning electron microscopy
LowVac = low vacuum
HiVac = high vacuum
T = tags of the dentine adhesives
D = dentine
A = dentine adhesives
H = hybride layer
asterisk = dentine tubule
This Poster was submitted by Prof. MUDr. Martina Kukletová.
Prof. MUDr. Martina Kukletová
CSc., Stomatological Clinic, Medical Faculty
656 91 Brno