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International Poster Journal of Dentistry and Oral Medicine
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Int Poster J Dent Oral Med 6 (2004), No. 2     15. June 2004

Int Poster J Dent Oral Med 2004, Vol 6 No 02, Poster 228

A CD-14 Polymorphism and Periodontitis in a South-German Population

Language: English

Authors:
Priv.-Doz. Dr. med. dent. Christof Dörfer,
Dr. med. dent. Holger Meiser,
Poliklinik für Zahnerhaltungskunde,
Dr. med. Christoph Lichy,
Priv.-Doz. Dr. med. Armin J. Grau,
Neurologische Klinik,
Universität Heidelberg

Date/Event/Venue:
September, 25-28th, 2002
Jahrestagung der CED
Cardiff/Wales

Objectives

The monocyte lipopolysaccharide receptor (LPS) CD14 plays an important role in the inflammatory response by mediating activation of macrophages, which results in the release of several inflammatory cytokines, including TNF-alpha and interleukin 1 and 6. Recently a polymorphism of the CD14 gene, C(-260)→T, was detected to be associated with several chronic inflammatory diseases.

Material und Methods

Subjects and Methods

151 consecutive patients with acute ischemic stroke (T) and 149 representative population controls (C) from a case control study were screened for the presence of the C(-260)→T polymorphism by restriction length analysis.

Subjects were clinically and radiographically examined for the presence of periodontitis (panoramic radiographs, number of teeth, caries, restorations, GI, PI, probing pocket depths, clinical attachment levels, furcation defects).

All individuals were interviewed by trained interviewers using a standardized questionnaire that focused on previous diseases, vascular and periodontal risk factors, including smoking, drinking habits and nutrition, social history, previous and present medication, and a detailed assessment of dental care.

Statistical Analysis

Data were included for all subjects who completed the study. The univariate analysis compared the amount of clinical attachment loss between TT-genotype and either CT- or CC-genotypes. The multivariate analysis calculated the Odds ratios of the TT-genotype for the presence of periodontitis according to the definition of Beck (1996). The statistical unit was the single subject. The significance level was set at p = 0.05 by the means of a multivariate logistic regression analysis including the parameters "age", "number of teeth", "diabetes mellitus", "smoking" and "socio-economic status". All analysis were done for the whole group and separate for male subjects under the age of 60 years.

Fig. 1 Example for the agarosis gel electrophoresis. Every column re-presents one subject.

Results

Descriptive data are shown in Table 1.

Subjects genotype teeth lost PI PPD CAL bone loss
all CT or CC 10.3 ± 9.2 1.53 ± 0.52 3.11 ± 0.67 3.88 ± 1.09 3.31 ± 1.65
TT 10.4 ± 9.4 1.67 ± 0.51 3.09 ± 0.67 4.02 ± 1.41 3.38 ± 1.72
p n.s. n.s. n.s. n.s. n.s.
male CT or CC 9.9 ± 9.9 1.58 ± 0.53 3.12 ± 0.71 3.93 ± 1.10 3.43 ± 1.77
TT 11.2 ± 9.7 1.76 ± 0.51 3.25 ± 0.70 4.33 ± 1.40 3.82 ± 1.86
p n.s. n.s. n.s. n.s. n.s.
≤ 60 CT or CC 7.3 ± 7.6 1.39 ± 0.46 3.00 ± 0.63 3.63 ± 0.99 3.03 ± 1.46
TT 7.9 ± 9.3 1.55 ± 0.50 2.98 ± 0.63 3.63 ± 1.16 2.99 ± 1.56
p n.s. n.s. n.s. n.s. n.s.
male ≤ 60 CT or CC 7.3 ± 8.1 1.43 ± 0.49 3.06 ± 0.69 3.69 ± 0.99 3.13 ± 1.55
TT 8.2 ± 9.5 1.62 ± 0.53 3.12 ± 0.67 3.91 ± 1.25 3.29 ± 1.73
p n.s. n.s. n.s. n.s. n.s.
Tab. 1 Descriptive statistics (mean value ± standard deviation).

By the means of a multiple logistic regression analysis in male subjects the presence of the C(-260)→T genotype of the CD14 gene resulted in a 3.39-fold higher risk of periodontitis (95%-CI: 1.02 - 11.23).

Fig. 2 Multivariate logistic regression model in male subjects on the association between periodontitis and the C(-260)→T genotype of the CD14 gene including systemic, socio-demographic and local risk factors for periodontitis. Odds ratios and 95%-confidence intervals are listed.

Fig. 3 Reduced multivariate logistic regression model in male subjects ≤ 60 years of age.

Discussion and Conclusions

Although the Odds ratios are statistically significant and independent from all known or suspected risk factors for periodontitis these results have to be confirmed by larger studies.

This poster was submitted by Priv.-Doz. Dr. med. dent. Christof Dörfer.

Korrespondenz-Adresse:
Priv.-Doz. Dr. med. dent. Christof Dörfer
Poliklinik für Zahnerhaltungskunde
Im Neuenheimer Feld 400
69120 Heidelberg
Germany