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International Poster Journal of Dentistry and Oral Medicine



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Int Poster J Dent Oral Med 3 (2001), No. 1     15. Mar. 2001

Int Poster J Dent Oral Med 2001, Vol 3 No 1, Poster 58

Bond strength of five current adhesives to caries-affected dentin

Language: English

Author(s): Abdulkadir Sengün, Nymet Ünlü, Füsun Özer, Bora Öztürk
University of Selçuk, Faculty of Dentistry, Konya, Türkiye

CONSERO 2000, Accademia Italiana Di Conservativa VII Congress
Bologna, ITALY


All published reports on the performance of bonding systems have used normal dentin as the substrate, even though caries-affected and sclerotic dentin are often the clinically relevant bonding substrates. Little is known about the effects of different conditioner, etchant, and self-etching systems on the bond strengths of resin to caries-affected dentin. The structural or physical characteristics of the caries-affected collagen fibrils that are exposed by etching with different etchants may be different from that of normal dentinal collagen fibrils. The spaces between the collagen fibrils in normal dentin are occupied by normal calcium-deficient, carbonate-rich apatite (LeGeros 1991). In caries-affected inter-tubular dentin, the mineral occupying the interfibrillar spaces may be different from that of normal apatite due to cyclic demineralization-remineralizations. Indeed, the Knoop Hardness of caries-affected dentin indicate that it is softer, even though many of the tubules are occluded with mineral crystallites (Ogawa et al 1983, Nakajima et al 2000). It is theoretically possible for adhesive resin monomers to penetrate further into less mineralized, more porous demineralized dentin but not wet the collagen fibers very well. The complete penetration of resin monomers in to the demineralized dentin is essential to create strong adhesion as well as a perfect seal of the enveloped collagen fibers (Sano et al,1995). For normal dentin, many reports are available with regard to both the tensile properties and interfacial morphology between the resin and the hybrid layer. Little high-resolution SEM has been reported on the interfacial morphology of the junction between adhesive resin and caries-affected dentin and/or sclerotic dentin. Tagami (1999) showed morphological variations in the resin-impregnated layer that were dependent on whether the dentinal tubules were occluded or opened. The mineral phase of carious dentin is remodeled by repeated sequences of demineralization and remineralization, which usually produce occlusion of the tubules with mineral crystals.


The purpose of this study was to test the hypothesis whether bonding to caries affected dentin will yield bond strengths that are lower than bonds made to normal dentin.

Material and Methods

Specimen preparation
Sixty extracted human molars with aproximal dentin caries extending approximately half way through the dentin were used in this study within one month of extraction. All teeth were stored at room temperature in physiological saline. The root of teeth were mounted in a 1,5x1,5 cm quadrangle mold using chemically cured acrylic resin. The teeth were mounted so that their long axis were perpendicular to the base of molds. The aproximal surfaces with and without caries were prepared parallel to the long axis of the teeth to expose a flat dentin surface by using an Isomet saw under water (Buehler Ltd, Lake Bluff I L). For caries affected dentin, grinding was performed using the combined criteria of visual examination and staining with a caries detector solution (Kuraray Co, Ltd, Osaka, Japan).

Bond strength analysis
The teeth were randomly divided in to five groups, each containing 12 teeth. Five commercially available adhesive systems and their resin composite materials were used (Table1).

The flat dentin surfaces of teeth were hand-polished with 600 grit silicon carbide abrasive paper under running water. The surfaces were examined after polishing to ensure that its orientation was not altered. After the completion of the bonding procedures, according to the manufacturers' instructions (Table 1, Figure 1) composite resins were added to the surface by packing the material into cylindrical shaped plastic matrixes with an internal diameter of 2.5 mm and height of 3 mm. Excess composite was carefully removed from periphery of matrixes with an explorer. Bonding agents and composites were cured with an HILUX Curing Light for required seconds. The intensity of light was 400 mW/cm2 at least. Specimens were then stored in distilled water at 37°C for 10 days before bond strength testing.

Figure 1: The materials used in the study

Table 1. Manufacturers, components and application procedures of the dentin bonding systems used in the study

For shear bond testing, the specimens were mounted in a universal testing machine (Testometric Micro 500, England). A Knife-shaped apparatus attached to a compression load cell and traveling at a cross head speed of 1 mm/min was applied to each specimen at interface between tooth and composite until failure occurred. The maximum load (N) was divided by the cross-sectional area of the bonded composite posts to determine shear bond strength in Mpa.

Fracture analysis
Fracture analysis were performed using an optical stereomicroscope (Olympus SZ4045 TRPT, Japan). Failures were classified as: cohesive if more than 80 % of resin was found remaining on the tooth surface, adhesive if less than 20 % of the resin remained on the tooth surface, or mixed if certain areas exhibited cohesive fracture while other areas exhibited adhesive fracture.

Statistical Analysis
The data were analyzed by Kruskal-Wallis one way analysis of variance: Mann-Whitney U multiple comparison test was used to determine specific differences between groups. Wilcoxon Rank Tests were used to test shear bond strength differences in between caries-affected and normal dentin in the same tooth.


The results of the shear bond testing were summarized in Table 2 and Figure 2. For different types of bonding systems, ANOVA analysis showed significant differences in mean values of bond strengths for both caries-affected (p=0.020) and normal dentin (p=0.000). Among the materials applied to caries-affected dentin, CSE, SB, and EP showed higher bond strength than OCB and PB (p< 0.05). SB, EP, and OCB showed statistically similar values (p<0.05). Among the materials applied to normal dentin, CSE had the highest bond strength (p<0.05). Also, SB and EP showed higher bond strength than OCB and PB (p<0.05).

Figure 2. Mean shear bond strength values of materials (MPa) to caries-affected (CAD) and normal dentin (ND)

CSE, SB, and EP had bond strengths which were not much different for caries-affected and normal dentin (p>0.05). However, bond strength of OCB and PB to caries-affected dentin were significantly higher than to normal dentin (p<0.05).

Table 2. Mean shear bond strength values of materials (MPa) to caries-affected and normal dentin

Table 3. Modes of failure

In general, a greater percentage of the fractures were adhesive at the tooth-composite junction. The fracture patterns of the specimens of the materials involved are given in Table 3. The macroscopic mode of failure for bonding systems SB, OCB, PB and EP appeared to be adhesive in nature. That is the bond failed in the dentin/composite interface. With system CSE, most of the failures appeared to be cohesive failure of the composite and dentin, although about 33% of the bond failures showed some adhesive failure and about 21% of the bond failures showed some mixed failure (Figure 3a, b, c).

Figure 3a. Fracture surface from shear bond test with SB Bond showing cohesive failure in CAD

Figure 3b. Fracture surface from shear bond test with SE Bond showing mixed failure in ND Figure 3c. Fracture surface from shear bond test with PB Bond showing adhesive failure in ND

Discussion and Conclusions

In this report, the shear bond strengths of CSE, SB, EP, OCB, and PB (2.495.38, 21.499.15, 21.199.17, 17.439.78, and 14.107.94 MPa respectively) to caries affected dentin and (29.918.95, 21.175.41, 17.456.21, 11.9910.15, and 10.574.56 MPa respectively) to normal dentin indicate that the later two system were more sensitive to caries affected dentin than the former three system. The results of this study indicated that there was no significant difference between the bond strength obtained on normal and caries affected dentin.
Sano et al (1995) tested the hypothesis that resin infiltration of demineralized dentin can restore its tensile properties to those of mineralized dentin. The results of their study indicate that some adhesive resins, after infiltrating demineralized dentin, can restore and even exceed the ultimate tensile strength of mineralized dentin.
Nakajima et al (1995) tested whether bonding to caries-affected dentin is inferior to bonding to normal dentin, and that the quality of the hybrid layer plays a major role in creating good adhesion. Bonding to normal dentin with either All Bond 2 (26.9±8.8 MPa) or Clearfil Liner Bond II (29.5±10.9 MPa) showed tensile bond strengths higher than those to caries-affected dentin (13.0±3.6 MPa and 14.0±4.3 MPa, respectively). The tensile bond strengths obtained with Scotchbond Multi-Purpose were similar in normal and caries-affected dentin (20.3±5.5 MPa and 18.5±4.0 MPa, respectively).
In another recent study, it was found that Scotchbond Multi-purpose Plus the bond strengths to dry, normal dentin were only half as strong (21±10 MPa) as those made to moist, normal dentin (42±9 MPa). There was no significant difference between bonds made to normal vs. caries affected dentin by means of the moist technique (42±9 vs. 48±4 MPa, respectively) (Nakajima et al 1999).
There is concern that some acidic conditioners may not be strong enough to adequately etch sclerotic or caries-affected dentin. Nakajima et al (2000) found that with One-Step following etching with 10% phosphoric acid showed lower (p<0.05) tensile bond strengths to caries-affected dentin compared to normal dentin (36.9±8.0 MPa vs 47.7±6.5 MPa respectively). This difference disappeared when using 32% phosphoric acid (49.7±6.1 MPa vs 45.0±7.2 MPa respectively). Bonds made to caries-affected dentin with Single bond were always lower than bonds to normal dentin regardless of the strength of the phosphoric acid.
In this study, even though two one-bottle bonding systems performed better bonding values on the caries effected dentin than they performed on the normal dentin. Both total-etch and self-etch bonding systems, which had similar values on caries affected and normal dentin, showed higher bond strength values than one-bottle systems. These results indicate that both total etch and self etch bonding systems were more successful on both normal and caries affected dentin than one-bottle systems.

The type of dentin and its histologic structure play an important role during bonding performance of adhesive resin materials. And also chemical composition of a bonding system can alter bonding mechanism.


  1. LeGeros R.Z. (1991).Calcium phosphates in oral biolgy and medicine. Vol. 15. Monographs in oral science. Myers H, editor. New York: Karger, pp.109-110.
  2. Nakajima M. Sano H. Urabe I. Tagami J. Pashley D.H. (2000) Bond strentghs of Single-Bottle Dentin adhesives to Caries-Affected dentin. Operative Dentistry 25:2-10.
  3. Nakajima M. Sano H. Zheng L. Tagami J. Pashley D.H. (1999). Effect of moist vs. Dry bonding to Normal vs Caries affected Dentin with Scotchbond Multi-purpose Plus. J Dent Res 78(7): 1298-1303.
  4. Nakajima M. Sano H. Burrow M.F. Tagami J. Yoshiyama M. Ebisu S. Ciucchi B. Russell C.M. Pashley D.H. (1995). Tensile bond Strength and Sem Evaluation of caries-affected Dentin using dentin adhesives. J Dent Res74(10): 1679-1688.
  5. Ogawa K. Yamashita Y. Ichijo T.Fusayama T. (1983). The ultrastructure and hardness of the transparent layer of human carious dentin.. J Dent Res 62:7-10.
  6. Sano H, Takatsu T. Ciucchi B. Russell C.M. Pashley D.H.(1995) Tensile properties of resin infiltrated demineralized human dentin. J Dent Res 74(4):1093-1102.
  7. Tagami J. (1999). New trend of adhesive restoration with Clearfil Liner Bond 2V. J. Morita Int. News 18(45): 1-7.


Bis GMA : bisphenyl-glycidyl-methacrylate
HEMA: 2-Hydroxyethyl methacrylate
MDP: 10-Methacryloyloxydecyl dihydrogen phosphate
HPMA: Hydroxypropylmethacrylate
PENTA: Pentaacryloyldipentaerythrytol phosphoric acid
TEG-DMA: triethyleneglycol dimethacrylate

This Poster was submitted on 03.02.01 by Dr. Abdülkadir Sengün.

Correspondence address:
Dr. Abdülkadir Sengün
Selcuk Üniversitesi Dis Hekimligi
Fakültesie, Dis Hastalikari ve Tedavisi A.D
Tedavi Bölmünü 42079, Kampüs/Konya