Int Poster J Dent Oral Med 20 (2018), No. 4 15. Dec. 2018
Int Poster J Dent Oral Med 20 (2018), No. 4 (15.12.2018)
Poster 1241, Language: English
Evaluation of Oral Potentially Malignant Disorders with Autoflorescence, Reflectance Spectroscopy, and Vital Staining and Their Correlation with Histopathology - a Hospital-based Pilot Study
Sharma, Deepa / Rimal, Jyotsna / Maharjan, Iccha Kumar / Shrestha, Ashish / Shrestha, Ashish / Regmee, Pragya / Luitel, Abhinaya / Adhikari, Sagar
Background: Oral squamous cell carcinoma is frequently preceded by clinical Oral Potentially Malignant Disorders (OPMDs) that correspond with an increased risk of cancer. Early recognition and diagnosis of OPMDs by means of screening can help in early diagnosis and, hence, improve patient survival, reduce treatment-related morbidity, and improve quality of life. The purpose of this study is to evaluate OPMDs with autofluorescence, reflectance spectroscopy, and vital staining and correlation with histopathology.
Materials and Methods: Patients with OPMDs visiting the Department of Oral Medicine and Radiology BPKIHS were included in the study. After oral examination, screening was done by Identafi®, followed by toluidine blue staining, and then screened again by Identafi®.Then, incisional biopsy under local anesthesia was performed from the site showing positive change. Lesions that display a loss of autofluorescence (LOA) were considered to be positive, and lesions that display no LOA were considered negative. Lesions with diffuse vasculature were considered to be positive, and lesions without visible vasculature or with vasculature similar to the surrounding mucosa to be negative.
Results: Of 10 patients (13 lesions), toluidine blue staining was positive in 46.15% (6 cases) of the cases and negative in 58.33% (7 cases). Diffuse vasculature was seen in 46.2% (6 cases), and vascularity was not appreciated in 53.84% of cases (7 cases). Identafi's violet light showed sensitivity of 85.7% and specificity of 50%.
Conclusion: Identafi® is highly sensitive in detecting true positive cases. The sensitivity of white, violet, and green light are 100%, 85.7%, and 100% and specificity 100%, 50%, and 76.9%, respectively. The sensitivity and specificity of toluidine blue obtained was 66.66% and 100%. Since this is a pilot study, there is a need for further samples before making any concrete inference. The screening of OPMDs will help in early diagnosis and further help in selecting the biopsy site followed by proper early intervention, thereby increasing the prognosis, and outcome and decreasing complication associated with it.
Keywords: Oral potentially malignant disorders, autofluorescence, reflectance spectroscopy, toluidine blue
Poster Award: First prize on E-poster presentation
First lecture and Workshop on Oral Medicine and Radiology, Theme: Advances in Oral Diagnostics
11.-12. May 2018