Int Poster J Dent Oral Med 19 (2017), OMD/PDA 15. Jan. 2018
Int Poster J Dent Oral Med 19 (2017), OMD/PDA (15.01.2018)
Supplement, Poster 1156, Language: English
Minocycline-releasing PMMA for craniofacial bone reconstruction
In vitro characterization
Silva, Tiago / Grenho, Liliana / Barros, Joana / Silva, José / Pinto, Rosana / Colaço, Bruno / Fernandes, Helena / Bettencourt, Ana / Gomes, Pedro
Introduction: Craniofacial traumatic lesions frequently lead to debilitating and challenging consequences, as a result of infectious processes which make the immediate and successful surgical regenerative treatment difficult and/or impossible.
Objectives: In the present work, it is aimed the development and biological characterization of a poly methyl methacrylate (PMMA)-based minocycline delivery system, to be used as a space maintainer within craniofacial staged regenerative interventions.
Materials and methods: Developed PMMA systems were characterized regarding solid state characteristics and assayed in vitro for anti-bacterial and anti-inflammatory activity, and cytocompatibility with human bone cells.
Results: Drug release profile revealed the maintenance of minimum inhibitory concentrations against Staphylococcus aureus, slime-producer-S. epidermidis and Escherichia coli, up to 72 hours. Further, controlled minocycline release decreased the release of pro-inflammatory mediators from activated macrophages and enhanced the functionality of osteoblastic cells, within in vitro models.
Discussion: PMMA-based minocycline delivery system allowed for an adequate drug release profile, supporting a sustained antibacterial activity against relevant pathogenic bacteria commonly associated with craniofacial bone infections. Further, a sustained anti-inflammatory activity and enhanced bone cell response were verified.
Conclusions: The developed PMMA systems, with controlled release of minocycline, allowed for an effective antibacterial activity and enhanced cytocompatibility, supporting a prospective utility for staged craniofacial reconstructive approaches.
Clinical implications: The developed PMMA system displays an effective antibacterial activity and expectedly primes tissue healing through inflammation modulation and enhancement of osteoblastic response.
Funding: This work was partly supported by the FCT grant UID/DTP/04138/2013.
Keywords: minocycline, bone, PMMA, drug delivery system, biological characterization and cytocompatibility
Poster Award: Best investigation poster
26th Annual Meeting of the Portuguese Dental Association 2017 (OMD)
16.-18. November 2017
Altice Arena, Lisbon, Portugal