Int Poster J Dent Oral Med 11 (2009), No. 3 15. Sep. 2009
Int Poster J Dent Oral Med 11 (2009), No. 3 (15.09.2009)
Poster 459, Language: German
Auswirkung verschiedener Gestagen-Kombinationen auf humane Endothelzellen
Nolting, Tim/Nolting, Lothar/Joos, Ulrich/Kleinheinz, Johannes
A sucessfull integration of a bone transplant gained from tissue engineering depends largely on a sufficient angiogenesis. While the effect of severel substances on angiogenetic processes has been studied thoroughly, the influence of hormonal anticontraceptives (OC) like estrogen an gestagen has so far been neglected. On grounds of the increasing use of natural and synthetic contraceptives the aim of this study was the examination of the the influence of different gestagen derivates in oral contraceptives on the proliferation of human endothelial cells.
Therefore human umbilial endothelisl cells (HUVEC) were gained using standard techniques and incubated with different combnations of ethinyl-estrogen (EE), gestagen and EE plus gestagen. The gestagen were 3 nortestosteron derivates (norethisteron-acetate, levonorgestrel, desogestrel) and one 17-alpha-hydroxyprogesteron derivat (Chlormadinon-Acetat) applied in 1 hour aquivalent doses. After 24 and 72 hours the proliferation of the endothelial cells was controlled photmetrically in size and ammount.
The results showed that oral contraceptives (OC) have an effect on angiogenesis. While some contens can increase the angiogenetic process others block it. The used progesterone derivate seemed to have an different effect on the endothelial cells.
All tested combinations had an decreasing effect on the cell number,only EE and EE+ norethistosterone acetate showed larger number in the cell count compared to the control.
Apart from EE with smaler cell diameter, all other combinations showed an increased diameter of the endothelial cells, in comparison with the control group.
As only a few gestagens and their combination could be investigated in this study further investigation have to enlarge the ammount of data. Anyhow the study showed thatOC have an influence on angiogenesis which can effect incorporation of implants and healing processes.
Keywords: angiogenesis, oral contraceptives, ethinyl estrogen, norethistosterone acetate, levonorgestrel, desogestrel
58. Jahrestagung Arbeitsgemeinschaft Kieferchirurgie