International Poster Journal of Dentistry and Oral Medicine
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Int Poster J Dent Oral Med 13 (2011), No. 1     15. Mar. 2011

Int Poster J Dent Oral Med 2011, Vol 13 No 1, Poster 522

Electron spin resonance spectroscopy (ESR) of albumin for diagnosis of oral squamous cell carcinoma – a pilot study

Language: English
 

Authors:
Dr. Dr. Peer W. Kämmerer, Dr. Dr. Maximilian Moergel, Dr. Dr. Marcus Oliver Klein, Andreas Bemsch, Prof. Dr. Dr. Bilal Al-Nawas,
Klinik für Mund-, Kiefer- und Gesichtschirurgie, Universitätsmedizin Mainz, Germany
Dr. Kerstin Schnurr,
MedInnovation GmbH, Wildau, Germany

Date/Event/Venue:
25.-29. Mai 2010
60. Kongress der Deutschen Gesellschaft für Mund-, Kiefer- und Gesichtschirurgie
München
 

Poster Award
Poster-Award for the best poster of the congress

Introduction

Albumin is known to play an important role for transportation of various proteins, peptides and low molecular weight molecules produced by tumor cells. The novel technique of spin labelling followed by electron spin resonance spectroscopy (ESR) is a tool to evaluate the structural and functional changes that can occur to albumin following the binding of various ligands.
 

Objectives

A difference of albumin functionality between cancer patients and healthy persons has been described in other tumor entities. Therefore, the present study investigated the value of ESR analysis for albumin-bound molecules as a possible biomarker in oral squamous cell carcinoma (OSCC).
 

Material and Methods

ESR was tested blinded to the clinical data. Peripheral blood was isolated from 32 patients with histological verified OSCC (m=18, w=14; T1 n=11, T2 n=10, T3 n=6, T4 n=5; N0 n=24, N1 n=6) and tested against 30 healthy volunteers (age and gender matched). A discriminant variable (DR) was calculated. Obtained data were associated with pathological and clinical data.

Fig. 1: Processing of samples (From: MedInformation GmbH) Fig. 2: Tumor cells are secreting proteins as well as protein fragments into the peripheral blood. These are binding to albumin and this results in a changed fatty acid binding. This changed fatty acid binding can be measured via electron spin resonance spectroscopy (ESR; form: MedInnovation GmbH).
 

Results

The analysis revealed a diagnostic sensitivity in detection of OSCC of 72% (23/32 patients with OSCC) and a specificity of 80% (24/30 healthy volunteers). A sub-group analysis with patients without leucocyte elevation (52 patients (84%; OSCC-group n=23, healthy volunteers n=29) was conducted. Here, sensitivity added up to 87% (20/23), specificity to 83% (24/29). In both groups, DR was significant different (p < 0.001) between OSCC- and volunteer group. ESR measurement "detected" all T4- as well as all N-1 stadiums.
 

Conclusions

High sensitivity and specificity implies a promising approach towards detection of OSCC by peripheral blood analysis. Nevertheless, inflammation seems to be a major confounder. As OSCC is often accompanied with inflammation, the method may be not appropriate in these cases. Prospective studies with higher patient numbers and an optional combination with other biomarkers are needed to confirm the value of ESR in OSCC diagnosis.
 

Literature

  1. Gurachevsky A, Kazmierczak SC, Jorres A, Muravsky V. Application of spin label electron paramagnetic resonance in the diagnosis and prognosis of cancer and sepsis. Clin Chem Lab Med 2008; 46: 1203-1210
  2. Lowenthal MS, Mehta AI, Frogale K, Bandle RW, Araujo RP, Hood BL, Veenstra TD, Conrads TP, Goldsmith P, Fishman D, Petricoin EF 3rd, Liotta LA. Analysis of albumin-associated peptides and proteins from ovarian cancer patients. Clin Chem. 2005 Oct;51(10):1933-45.
     

This Poster was submitted by Dr. Dr. Peer W. Kämmerer.
 

Correspondence address:
Dr. Dr. Peer W. Kämmerer
Universitätsmedizin Mainz, Klinik für Mund-, Kiefer- und Gesichtschirurgie
Augustusplatz 2
55131 Mainz
Germany