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Int Poster J Dent Oral Med 10 (2008), No. 4     15. Dec. 2008

Int Poster J Dent Oral Med 2008, Vol 10 No 04, Poster 425

C-6-S Levels in PMICF under Orthodontic Forces

Language: English
 

Authors:
Dr. Imjai Intachai, Assoc. Prof. Dhirawat Jotikasthira, Assoc. Prof. Boonsiva Suzuki, Dr. Eduardo Yugo Suzuki,
Department of Orthodontics, Faculty of Dentistry
Assoc. Prof. Suttichai Krisanaprakornkit,
Department of Odontology and Oral Pathology, Faculty of Dentistry
Assoc. Prof. Prachya Kongtawelert,
Thailand Excellence Center for Tissue Engineering, Department of Biochemistry, Faculty of Medicine
Chiang Mai University, Thailand.

Date/Event/Venue:
December 14-16, 2007
The 6th Asian Implant Orthodontic Conference (AIOC) & TAO Annual Conference
Nan Shan Educational & Training Center, Taichung, Taiwan

Poster Award
The second place of the outstanding presentations
 

Introduction

Stability assessments of the miniscrew implant were previously based on stability assessments of dental implant. Biochemical assessments were widely used to assess stability of dental implant, but rarely for the miniscrew implant.
 

Material and Methods

A total of ten orthodontic patients were included in this study. Twenty miniscrew implants (8.0 mm in length with 1.6 mm in diameter, Sin, Brazil) were used as orthodontic anchorage. This experiment consisted of two parts. Part I was five weeks and Part II was ten weeks.

Fig. 1: Experimental design
 

Results

Part I

I. Clinical observations
• Unloaded period (1 week) : All miniscrew implants remained clinically immobile.
• Loaded period (4 weeks) : Two miniscrew implants were mobiled.

II. Chondroitin sulfate epitope (WF6 epitope) levels in PMICF samples
• The median of CS epitope (WF6 epitope) levels during loaded period was significantly greater than that during unloaded period (P < .05)

Fig. 2: Medians of C-6-S levels (ng/ml) in PMICF samples during unloaded period (n = 40) and loaded periods (n = 38).
 
Fig. 3: The median of C-6-S levels during loaded periods was significantly greater than that during unloaded period (P < .05).
 

Part II

I. Clinical observations
• Unloaded period (1 week) : All miniscrew implants remained clinically immobile.
• Loaded period (9 weeks) : One miniscrew implant was mobiled.

II. Chondroitin sulfate epitope (WF6 epitope) levels in PMICF samples
• No significant difference was found between the medians of CS epitope (WF6 epitope) level during unloaded and loaded period.

Fig. 4: Medians of CS epitope (WF6 epitope)levels (ng/ml) in PMICF samples during unloaded period (n = 40) and loaded periods (n = 82).
 
Fig. 5: No significant difference was found between the medians of C-6-S level during unloaded and loaded period.
 

Conclusions

  • The CS level in peri-dental implant crevicular fluid has been used for monitoring bone resorption and health status of dental implant.(1,2,3)
  • In our previous study(4), the CS epitope (WF6 epitope) detected in GCF around canine teeth could be used as biomarker of alveolar bone resorption under orthodontic forces.
  • The results of our present study showed that the CS epitope (WF6 epitope) in PMICF both under and without orthodontic loaded could be precisely detected. These findings were coincided with those of Sari and Uçar (5) that IL-1β levels (a peri-miniscrew implant alveolar bone resorption maker) could be precisely detected.
  • The ELISA method provides an accurate assessment of CS epitope (WF6 epitope) level with the simplicity of a "chairside" application.
  • To draw a reasonable conclusion for using the CS epitope (WF6 epitope) level as a biomarker for assessing alveolar bone remodeling around miniscrew implants, further investigation with an increased sample size is needed.
     

Literature

  1. Boutros SM, Michalowicz BS, Smith QT, Aeppli DM. Crevicular fluid enzymes from endosseous dental implants and natural teeth. Int J Oral Maxillofac Implants 1996;11:322-330.
  2. Beck CB, Embery G, Langley MS, Waddington RJ. Levels of glycosaminoglycans in peri-implant sulcus fluid as a means of monitoring bone response to endosseous dental implants. Clin Oral Implants Res 1991;2:179-185.
  3. Last KS, Smith S, Pender N. Monitoring of IMZ titanium endosseous dental implants by glycosaminoglycan analysis of peri-implant sulcus fluid. Int J Oral Maxillofac Implants 1995;10:58-66.
  4. Jaito N, Jotikasthira D, Krisanaprakornkit S, Ong-chai S, Kongtawelert P. Monotoring of chrondroitin-6-sulfate levels in gingival crevicular fluid during orthodontic canine movement. Biological Mechanisms of Tooth Eruption, Ressorption and Movement 2006:289-295.
  5. Sarı E, Uçar C. Interleukin 1b Levels around Microscrew Implants during Orthodontic Tooth Movement. Angle Orthod 2007;77:1073-1077.
     

This Poster was submitted by Dr. Imjai Intachai.
 

Correspondence address:
Associate Professor Dhirawat Jotikasthira
Department of Orthodontics, Faculty of Dentistry
Chiang Mai University
Chiang Mai 50200
Thailand
Tel. +66 53 944465
Fax +66 53 222844
e-mail: dhirawat@chiangmai.ac.th