Int Poster J Dent Oral Med 2008, Vol 10 No 01, Poster 401
Histologic evaluation of healing process of bone defects with or without using of guide bone regeneration techniques - animal studies on rabbits
DDS Marzena Dominiak, Katarzyna Łysiak-Drwal,
Department of Oral Surgery, Silesian Piasts University of Medicine of Wroclaw,
Leszek Solski, Bogusława Żywicka, Stanisław Pielka,
Department of Experimental Surgery and Investigation of Biomaterials,
Department of Oral Pathology
10 - 12. 05. 2007
International Symposium Osteology
The purpose of the study is histologic evaluation of healing process of
the intraosseus defects in rabbits with or without using of GBR techniques
after 6 months. Obtained results will be useful to work out a treatment
pattern of the human intraosseus defects after apectomy or cystectomy. Main
difficulty in setting of this algorithm is to establish conditions in which
GBR is not necessary and defect will be filled with proper bone tissue.
However in cases which require GBR is essential to establish the way of
choice of optimal GBR technique on the basis of clinical conditions and
restrictions of using some kinds of biomaterials.
Material and Methods
12 adult white rabbits were used in this study. This animal study was
approved by the I Local Animal Experimentation Committee in Wrocław nr.
30\\05. Under general anesthesia a cutaneus incision (Figure 1) and blunt
muscles preparation was made. The periosteum was incised and lifted to
expose the thighbone- the operation area (trochanter major ossis femoris).
One hole (diameter of 5 mm) will be drilled using a round steel bur (Figure
2). The same procedure will be carried out on the second leg.
|Figure 1: Cutaneus incision
||Figure 2: Driling procedure
The rabbits were divided into three groups (three in each group) for the sake of the way
of intraosseus defects treatment.
I. (C) - control group with ID with no GBR
procedures (Figure 3).
II. (BOC+BG) - group with ID filled with Bio-Oss
Collagen (Geistlich, Switzerland) and covered Bio Gide Perio (Geistlich,
Switzerland) membrane (Figure 4).
III. (BOC+PRP) -group with ID filled with
Bio-Oss Collagen (Geistlich, Switzerland) in connection with PRP -
(Platelet- Rich Plasma) (Figure 5).
In the third group 4 ml of blood was
taken from an ear vein. The blood will be used to receive PRP (MPW 223 prod.
MPW Instruments + PRP-Kit) (Figure 6). The selected materials were inserted
into the hole of the bone (1 rabbit = 2 holes).
|Figure 3: First group- intraoperation view- infrabone defect with no GBR procedure
||Figure 4: Second group- intraoperation view- infrabone defect filled with Bio-Oss Collagen and covered Bio-Gide Perio membrane
|Figure 5: Third group- intraoperation view- infrabone defect filled Bio-Oss Collagen in connection with PRP
||Figure 6: Platelet Rich Plasma
|Figure 7: Centrifuge of MPW Instruments
||Figure 8: First stage of centrifugation
The animals were euthanized with an overdose of pentobarbital 6 month after surgical procedure (Figure
9-I group, Figure 10- II group, Figure 11- III group). The sections were
stained with hematoxylin and eosin and with van Gieson method. Qualitative
histologic parameters will be assessed: overall tissue health and degree of
inflammation and integration of the xenogenic bone mineral particles into
the host bone.
|Figure 9: Macroscopic view of first group 6 months after operation
||Figure 10: Macroscopic view of second group 6 months after operation
|Figure 11: Macroscopic view of third group 6 months after operation
The bone defect was filled with spongy bone, which
generally had mature and lamellar structure. Bone remodeling was observed in
a few spaces with characteristic narrow threads of the plexus bone. Bone
trabeculas were surrounded by marrow. The entrance to osseus defect was
filled from upper side with cortilaginous tissue, lamellar and plexus bone
with significant amount of blood vassels. There were no signs of
inflammation. Histologic markers of inflammation, eg. neutrophilis,
macrophages were not observed (Figure 12).
Group BOC+ BG
The bone defect was filled with spongy bone (lamellar and plexus bone) surrounded by
yellow and red marrow. The remains of biomaterial were observed. No evidence
of residual membrane components was found in any of the sections. There were
fewer quantity and more significant fragmentation of biomaterial in
comparison with the PRP group. Generally there were no histological signs of
inflammation (Figure 13).
Remaints of biomaterial
with surrounding spongy bone tissue were seen. In infrabone defect there is
much more bone trabeculas and they are thicker in comparison with control
group. Around every remains of material newly formed bone trabeculas or
trabeculas in forming stage were presened. Biomaterials in various phase of
resorption and fragmentation which was followed by change of pigmentation
and structure (since places with normal structures- to defragmentated
strongy alkali- change of pigmentation into strongly violet). Direct contact
biomaterial/plexus bone and subsequently lamellar bone. In rare cases the
material was surrounded by connective tissue. In some of the sections there
were clinical signs of inflammation (Figure 14).
|Figure 12: Histologic section of the bone defect 6 months after operation (magnification x 4, Van Gieson stain)
||Figure 13: Histologic section of the bone defect 6 months after grafting biomaterial Bio-Oss Collagen and Bio-Gide membrane (magnification x 20, Van Gieson stain)
|Figure 14: Histologic section of the bone defect 6 months after grafting biomaterial Bio-Oss Collagen with Platelet Rich Plasma (magnification x 40, Van Gieson stain)
Usage of guide bone regeneration controlled after six months post
augmentation caused increase of number of bone trabeculas in comparison with
the control group. Biomaterial osteointegration process in the second group
was faster with less intensity of inflammatory reaction than in the third
group, but in both cases this process was not finished.
Study partially suported by Osteology Foundation
This Poster was submitted by DDS Marzena Dominiak.
DDS Marzena Dominiak
Silesian Piasts University of Medicine of Wroclaw,
Department of Oral Surgery,
ul. Krakowska 26