International Poster Journal of Dentistry and Oral Medicine



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Int Poster J Dent Oral Med 8 (2006), No. 1     15. Mar. 2006

Int Poster J Dent Oral Med 2006, Vol 8 No 01, Poster 305

Herbal extracts as adjunct in supportive periodontal therapy

Language: English

Dr. Grindwit Sastravaha, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Dr. Georg Gassmann, Department of Periodontology, Faculty of Dental Medicine, University of Witten/Herdecke, Germany
Mr. Preecha Sangtherapitikul, Particle Technology Association of Thailand, Bangkok, Thailand
Prof. Dr. Wolf-Dieter Grimm, Department of Periodontology, Faculty of Dental Medicine, University of Witten/Herdecke, Germany

Jubiläumstagung der Deutschen Gesellschaft für Parodontologie e.V. (DGP) zum 80. Jahrestag der Gründung der ARPA gemeinsam mit der GZMK Dresden e.V.


Centella asiatica and Punica granatum are medicinal plants that have been reported to promote tissue healing and modulate host responses. Preliminary study (11) revealed positive clinical effects of an innovative preparation from the two herbal extracts in the form of biodegradable chips as a subgingival adjunct to scaling and root planing.

Fig. 1 Centella asiatica Fig. 2 Punica granatum


The purpose of this research was to evaluate further augmenting efficacy the combined herbal preparation may have among maintenance patients in comparison to standard supportive periodontal therapy (SPT), with additional monitoring of certain inflammatory markers.

Material and Methods

Fifteen patients in the recall programme who had completed conventional periodontal therapy with remaining probing pocket depth 5-8 mm were enrolled. After baseline examination and collection of gingival crevicular fluid (GCF) samples, SPT was provided and the target teeth in the test group received subgingival delivery of the medicated chips (Fig. 3). The clinical parameters which included probing pocket depth (PD), attachment level (AL), bleeding index (BI), gingival index (GI) and plaque index (PI) were recorded and GCF samples were collected at baseline, 3 and 6 months (Table 1) for analysis of immunological mediators using enzyme link immunosorbent assay (ELISA).

Fig. 3 Subgingival delivery of medicated chip Fig. 4 GCF collection


Visit Procedures
Screening exam PD, case selection, randomization
Baseline PD, AL, BI, GI, PI, GCF, SPT, chips
3. month follow-up PD, AL, BI, GI, PI, GCF, SPT, chips
6. month follow-up PD, AL, BI, GI, PI, GCF, SPT
Table 1: Summary of clinical procedures


The results showed significant improvement of PD, AL, GI at 3 and 6 months (Fig. 5-7) and BI at 6 months in the test group compared to control. No significant differences in PI were found between the two treatment modalities at all subsequent visits. The test group also showed statistically greater reduction of IL-1ß at both 3 and 6 months and lower IL-6 concentration which almost reached significant level at 6 months (Table 2).

Fig. 5 PD & AL improvement at 3 and 6 months for all PD Fig. 6 PD & AL improvement at 3 and 6 months for initial PD 7-8 mm


Fig. 7 Changes in mean GI at 3 and 6 months


Treatment IL-1β (pg/μl) p-value IL-6 (pg/μl) p-value
0 3 6 0-3 0-6 0 3 6 0-3 0-6
SPT alone 31.81±8.26 19.51±5.87 13.56±3.61 0.349 0.105 2.01±0.52 0.48±0.15 § 0.38±0.16 § 0.006 0.003
SPT+med 49.91±5.63 19.96±3.55 § 13.47±2.05 § < 0.001 < 0.001 1.49±0.28 0.19±0.07 § 0.04±0.03 § < 0.001 < 0.001
p-value 0.102 0.949 0.983 - - 0.386 0.078 0.055 - -
Table 2: Mean SD of IL-1ß and IL-6 levels at baseline, 3 and 6 months


Study Duration (months) Treatment Mean initial PD (mm) Mean PD reduction from baseline (mm) Mean AL reduction from baseline (mm)
Jacoby (1997)
6 SRP alone 5.57 1.50 -
metronidazole alone 5.65 1.32 -
2.Rudhart et al.
6 SRP alone 5.7 1.6 0.5
metronidazole alone 5.8 1.6 0.7
3.Garrett et al.
9 SRP alone 5.9 1.1 0.8
doxycycline alone 5.9 1.3 0.7
4.Heasman et al.
6 SRP alone 6.47 0.45 0.15
SRP+CHX chip 6.64 0.78 0.43
5.He et al.
6 SRP alone - 0.77 0.40
SRP+CHX chip - 1.32 0.94
6.Sastravaha et al.
6 SPT alone 6.21 0.36 0.12
SPT+med 6.39 1.02 0.48
Table 3: Mean PD&AL improvement from clinical trials employing different local adjunctive agents in supportive periodontal therapy


  1. The clinical improvement in SPT+med was confirmed by immunological mediator analysis. Together with other reports (7, 11, 12), the results from this study indicate that additional benefits may be obtained by utilizing certain botanically derived substances as adjunct in standard periodontal therapy.
  2. In contrast to the anti-infective phase, local antimicrobial delivery has not been widely used in maintenance patients. Some information, however, are summarized in Table 3 to allow comparison with other investigations (3, 5, 6, 10, 13). It may seem that SPT+med in the present trial yielded slightly less PD and AL. Partially, this may be due to the different kinds of adjunctive agents themselves. In addition, the method used in each study and inconsistence in the mean initial PD have to be taken into consideration as changes in PD or AL may differ between therapies (4). In this study, scaling and root planing was not included and the mean initial PD was also somewhat greater than those of most other reports.
  3. Presence of bleeding on probing is a good indicator to indicate presence of inflammation (9). SPT+med showed significantly better BI at 6 months and GI at 3 and 6 months. This reflects the capability of the herbal chips in reducing degree of inflammation and improving gingival health. PI, on another hand, was clearly seen as being independent from the treatment modalities with no significant differences at any time points.
  4. The use of local delivery systems with antimicrobials will not replace the necessity of scaling and root planing (8). Repetitive mechanical debridement, however, may result in loss of attachment (1) and tooth structure (16), possibly followed by dentin sensitivity (14). Moreover, patient compliance with maintenance visits is usually poor (15). Some patients also consider periodontal instrumentation to be too traumatic and less invasive alternatives would therefore be welcome (2). According to the above argument, local delivery with herbal extracts may serve as a reasonable compromised strategy to substitute mechanical instrumentation at some recall appointments and represent a new alternative for adjunctive agents, especially when selection of drugs in the antibiotic or NSAID classes is contraindicated or undesirable.
  5. Although the present study provided quite positive results which support a previous study (11), they are sufficient to answer questions to a certain extent. Adjustment of the methodology may be considered in a future trial including increasing the sample size for better extrapolation of the results. Extending the duration of follow-up period may also enable detection of further changes over time at both clinical and subclinical levels.


  1. Alves, R.V., Machion, L., Casati, M.Z., Nociti Jr., F.H., Sallum, A.W. and Sallum, E.A. Attachment loss after scaling and root planing with different instruments. Journal of Clinical Periodontology 2004; 31:12-15.
  2. Echeverria, J.J., Manau, C. and Guerrero, A. Supportive care after active periodontal treatment. A review. Journal of Clinical Periodontology 1996; 23:898-905.
  3. Garrett, S., Adams, D.F., Bogle, G., et al. The effect of locally delivered controlled-release doxycycline or scaling and root planing on periodontal maintenance patients over 9 months. Journal of Periodontology 2000; 71:22-30.
  4. Haffajee, A.D., Cugini, M.A., Dibart, S., Smith, C., Kent Jr., R.L. and Socransky, S.S. The effect of SRP on the clinical and microbiological parameters of periodontal diseases. Journal of Clinical Periodontology 1997; 24:324-334.
  5. He, L., Geng, S. and Cao, C. The efficacy of the chlorhexidine chip following scaling and root planing (SRP) and compared to SRP alone. Chinese Journal of Stomatology 2001; 36:443.
  6. Heasman, P.A., Heasman, L., Stacey, F. and McCracken, G.I. Local delivery of chlorhexidine gluconate (PeriochipTM) in periodontal maintenance patients. Journal of Clinical Periodontology 2001; 28:90-95.
  7. Hirasawa, M., Takada, K., Makimura, M. and Otake, S. Improvement of periodontal status by green tea catechin using a local delivery system: a clinical pilot study. Journal of Periodontal Research 2002; 37:433-438.
  8. Killoy, W.J. Chemical treatment of periodontics: local delivery of antimicrobials. International Dental Journal 1998; 48:305-315.
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  10. Rudhart, A., Purucker, P., Kage, A., Hopfenmüller, W. and Bernimoulin, J.P. Local metronidazole application in maintenance patients. Clinical and microbiological evaluation. Journal of Periodontology 1998; 69:1148-1154.
  11. Sastravaha, G., Yotnuengnit, P., Booncong, P. and Sangtherapitikul, P. Adjunctive Periodontal Treatment with Centella asiatica and Punica granatum Extracts. A Preliminary Study. Journal of the International Academy of Periodontology 2003; 5:106-115.
  12. Song, S.E., Choi, B.K., Kim, S.N., et al. Inhibitory effect of procyanidin oligomer from elm cortex on the matrix metalloproteinase and proteases of periodontopathogens. Journal of Periodontal Research 2003; 38:282-289.
  13. Stelzel, M. and Flores-de-Jacoby, L. Topical metronidazole application in recall patients. Journal of Clinical Periodontology 1997; 24:914-919.
  14. von Troil, B., Needleman, I. and Sanz, M. A systemic review of the prevalence of root sensitivity following periodontal therapy. Journal of Clinical Periodontology 2002; 29:173-177.
  15. Wilson, T.G., Glover, M.E., Schoen, J., Baus, C. and Jacobs, T. Compliance with maintenance therapy in a private periodontal practice. Journal of Periodontology 1983 55, 468-473.
  16. Zappa, U., Smith, B., Simona, C., Graf, H., Case, D. and Kim, W. Root substance removal by scaling and root planing. Journal of Periodontology 1991; 62:750-754.


This Poster was submitted by Dr. Grindwit Sastravaha.

Correspondence address:
Dr. Grindwit Sastravaha
Faculty of Dentistry
Mahidol University
352/1 Krungthonburi 4 Banglampoolang
Klongsan Bangkok 10600