Int Poster J Dent Oral Med 19 (2017), OMD/PDA 15. Jan. 2018
There is a lot of ambiguity in the armamentaria for PRF preparation, and these are frequently not fully disclosed or vary across individual studies. The standard protocol for L-PRF is running a centrifuge at 2700 rpm for 12 minutes at around 400g in tubes which have a fixed angulation of 33°. The G-force and RPM of a centrifuge are related by the formula: G-force = 1.12 X centre-of-centrifuge to tube distance X (RPM/1000)². However, there is extreme variability among centrifuges when it comes to tube angulation and the centre-of-centrifuge to tube distance. At 2700 RPM, the centrifugal force is clearly in excess of the 400g recommended in the literature. This may have detrimental effects on the PRF structure, composition, and density. Therefore, it is necessary to adjust the RPM in every centrifuge to generate precisely 400g of force for preparation of PRF using the above formula.
The objective of this study was to compare optical density (OD) and fibrinogen content of PRF by standard protocol vs an adjusted protocol (1650 RPM/12mins). Standard protocol was used in all three centrifuges (DUOS®, Remi 8C® and Remi C854®). The adjusted protocol was derived by initially measuring the centre-of-centrifuge to tube distance. By using the formula mentioned above, the ideal RPM to generate 400g of force was calculated in two commercially available laboratory centrifuges (Remi 8C® and Remi C854®). While, no significant differences were present across all centrifuges, laboratory centrifuges resulted in a denser clot and higher fibrinogen content over fixed angle centrifuge (DUOS®).
Keywords: PRF, L-PRF, Centrifuge, Fibrinogen, Spectrophotometry, Optical density
41st National Conference, Indian Society of Periodontology
21.-23. October 2016
Chitnavis Centre, Nagpur, Maharashtra, India