Int Poster J Dent Oral Med 14 (2012), No. 4 15. Dec. 2012
Int Poster J Dent Oral Med 14 (2012), No. 4 (15.12.2012)
Poster 621, Language: English
Antifungal Efficacy of 5·25 percent sodium hypochlorite , 2 percent chlorhexidine gluconate and 17 percent EDTA with and without antifungal agent as root canal irrigant
Wadhwani, Kulwinder Kaur / Gupta, Richa / Hasija, Mukesh
Aim: The aim of this invitro study was to evaluate the antifungal efficacy of 5·25 percent Sodium Hypochlorite, 2 percent Chlorhexidine Gluconate, and 17 percent EDTA as final irrigant with and without the inclusion of an Antifungal agent that is 1 percent Clotrimazole on Candida albicans.
Methods: 30 single rooted instrumented teeth were inoculated with the suspension of Candida albicans. These 30 samples were divided into TWO groups of 15 samples each. GROUP 1 - Without antifungal agent GROUP 2 - With antifungal agent that is 1 percent Clotrimazole. The GROUP 1 containing 15 samples was further subdivided into three subgroups of 5 samples each. Samples in subgroup 1 were irrigated with 5·25 percent sodium hypochlorite, samples in subgroup 2 were irrigated with 2 percent chlorhexidine gluconate, and samples in subgroup 3 with 17 percent EDTA. The GROUP 2 containing 15 samples which is with antifungal agent was similarly subdivided into three subgroups of 5 samples each and was similarly irrigated with 5·25 percent sodium hypochlorite, 2 percent chlorhexidine gluconate, and 17 percent EDTA respectively but was followed by 1percent clotrimazole as a final rinse. Then aliquots from these samples were plated on 4 percent sabouraud agar, and the colony forming units were evaluated.
Results: 5·25 percent sodiumhypochlorite exhibited superior antifungal efficacy compared to 2 percent chlorhexidine gluconate and 17 percent EDTA. On inclusion of 1 percent clotrimazole, there was a significant decrease in colony forming units. 5·25 percent sodiumhypochlorite and 2 percent chlorhexidine gluconate with clotrimazole showed significantly greater antifungal properties than 17 percent EDTA with clotrimazole.
Conclusion: 1 percent clotrimazole proved to be effective against Candida albicans when used along with root canal irrigants as a final rinse.
Keywords: antifungal, Candida albicans, chlorhexidine gluconate, clotrimazole, EDTA, irrigation, sodium hypochlorite
Twelfth November 2011
Twentysixth Federation of Operative Dentistry of India and nineteenth Indian Endodontic Society National Conference
Manekshaw Convention Centre, New Delhi, India