Int Poster J Dent Oral Med 5 (2003), No. 1 15. Mar. 2003
Int Poster J Dent Oral Med 5 (2003), No. 1 (15.03.2003)
Poster 162, Language: German
Apoptose sulkulärer PMNL bei Patienten mit Aggressiver Parodontitis in verschiedenen Sondierungstiefenkategorien
Raabe, Hagen/Sigusch, Bernd Wilfried/Vogelsang, Heinz/Klinger, Gisela/Glockmann, Eike
Crevicular PMNL's are the predominant cells in the local defense against periodontopathogenic bacteria. Ellis et al. 1996 reported that apoptosis occurs only on sites of periodontitis but not gingivitis. But until now, the proportion of apoptotic or necrotic cells in crevicular fluid is not examined enough. In this context, we investigate apoptotic and necrotic PMNL's in the crevicular fluid of patients with periodontal disease.
CF-cells from 29 patients with aggressive periodontitis were obtained by crevicular washings as we described recently.
Aggressive periodontitis patients were characterized by various pocket depth categories: 1-3,5 mm, 4-6 mm, 6,5-9 mm and >9 mm. Apoptosis of CF-cells was determined using the Annexin-V-Fluos Staining Kit (Boehringer Mannheim, Germany) and flow cytometry (Facs).
To identify apoptotic or dead neutrophils FITC-annexin V.and propidium iodide was used.We found that in periodontitis patients with pocket depth categories: 4-6 mm and 6,5-9 mm, 55% of the PMNL's were apoptotic, compared to the pocket depth categories: 1-3,5 mm and >9 mm(8% and 4%).
Furthermore, in category 1-3,5 mm in periodontitis patients we obtained a smaller number of necrotic cells compared to the category >9 mm (p9mm only a small number of apoptotic and furthermore in the last category, a greater number of necrotic CF-cells.
Keywords: aggressive Parodontitis, PMNL, Apoptose, Sondierungstiefenkategorien